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This laboratory and lecture
course is designed to provide neuroscientists at all levels
with a conceptual and practical understanding of several of
the most advanced techniques in molecular neuroscience. The
course curriculum is divided into three sections: an extensive
and up-to-date set of laboratory exercises, daily lectures
covering the theoretical and practical aspects of the various
methods used in the laboratory, and a series of evening research
seminars. The informal and interactive evening lectures will
be given by leading molecular neuroscientists and will serve
to illustrate the ways in which the various experimental approaches
have been used to advance specific areas of neurobiology.
In this year's course, the laboratory portion will include
topics such as: an introduction to the design and use of animal
virus vectors in neurobiology; the use of small interfering
RNAs (siRNA) for regulating the expression of specific genes
in neurons; practical exercises in gene delivery systems including
mammalian cell transfection protocols and single cell electroporation
techniques for targeted gene transfer in vivo; an introduction
to overall strategies, use and design of BAC transgenic vectors;
multiplex and whole genome expression analyses using the most
recent DNA microarray technologies (including labeled probe
preparation, data analyses, mining, and interpretation); quantitative
real time RT-PCR analyses from small numbers of cells (RNA
purification, PCR optimization, interpretation of results);
single cell PCR and cDNA library construction; methods and
application of RNA amplification (aRNA). Each laboratory module
will be followed by comprehensive data analyses and interpretation,
protocol troubleshooting, and suggestions for ways to improve
or modify the existing technique. Finally, course participants
will be introduced to bioinformatics and a wide range of internet
resources which are available to molecular neuroscientists.
Speakers in the 2011 course
included:
Ben Barres, Stanford University
School of Medicine
Thomas Bell, NDRI Resource
Robert Darnell, HHMI, Rockefeller University
Susan Dymecki, Harvard Medical School
James Eberwine, University of Pennsylvania Medical School
Lisa Gunaydin, Stanford University
Kurt Haas, University of British Columbia
Jeff Lichtman, Harvard University
Pierre-Marie Lledo, Pasteur Institute, France
Brady Maher, University of Connecticut
Kate Storey, University of Dundee College of Life Science
This
course is supported with funds provided by the National
Institute of Mental Health, the National
Institute of Neurological Disorders & Stroke and the
Howard Hughes
Medical Institute.
Equipment
and reagents generously provided by New England Biolabs Inc.;
Olympus America Inc.; Carl Zeiss Inc. along with Bitplane-Andor
Technology; Bio-Rad Laboratories; Chroma Technology Corporation;
Coherent Laser; Eppendorf North America; Fotodyne Inc.; Harvard
Apparatus Inc.; Illumina Inc.; Invitrogen Corporation; Leica
Microsystems Inc.; Nanodrop Technologies; Narishige International
USA Inc.; PerkinElmer Life; Promega Corporation; Qiagen Inc.;
Roche Applied Science; Sigma-Aldrich; Thermo Fisher Scientific.
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Scientists from developing countries
accepted into this course may be eligible for scholarships
provided by the International Brain Research Organization
(Click here
for details)
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Cost
(including board and lodging): $3,865
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